Freqüência dos antígenos de histocompatibilidade na populaçäo normal da regiäo nordeste do Estado de Säo Paulo Brasil / Frequency of histocompatibility antigens in the normal population of the northeast region of the State of Säo Paulo Brazil

Modelo de estudo: estudo de prevalência. Objetivos: avaliaçäo das freqüências dos antígenos de histocompatibilidade de classe I (HLA-A, -B e -C) e de classe II (HLA-DR e -DQ) na populaçäo de indivíduos saudáveis da regiäo nordeste do Estado de Säo Paulo. Metodologia: foram estudados três grupos de indivíduos saudáveis, o de funcionários do Hospital de Clínicas e da Faculdade de Medicina de Ribeiräo Preto, o de doadores do Banco de Sangue local e o de doadores cadáveres de órgäos sólidos do Säo Paulo Interior Transplante. Os antígenos HLA foram tipificados, utilizando-se método de microlinfocitoxicidade-dependente de complemento. Resultados: as freqüências dos antígenos de histocompatibilidade de classe I ou II, usualmente detectados entre os três grupos estudados, foram semelhantes. Conclusões: as populações de indivíduos saudáveis estudadas refletem adequadamente o perfil imunogenético da populaçäo da regiäo nordeste do Estado de Säo Paulo, podendo esses dados ser utilizados para estudos de seleçäo de doadores em transplantes, e, também, naqueles de associaçäo com doenças

HLA-DR and HLA-DQ typing: a comparative study using serology and restriction fragment length polymorphism (RFLP) analysis

Serology has been used for HLA typing for many decades; however, serological typing of histocompatibility class II molecules depends on the adequade expression of these molecules on the surface of B lymphocytes, the availability of viable cells and a complete set on antisera. HLA typing at the genomic level has supplanted these pitfalls. The utilization of restriction fragment length polymorphism (RFLP) was the first approach to the HLA typing at molecular level. Although serology and RFLP methods define HLA specificities at low resolution level, RFLP has been considered to be better than serology. In this study, we performed HLA classe II (HLA-DR and DQ) typing comparing these two methods

Immunogenetic features of steroid-sensitive nephrotic syndrome and focal segmental glomerulosclerosis in brazilian patients of the northeast region of the State of Säo Paulo

Steroid-sensitive nephrotic syndrome (SSNS) and focal segmental glomerulosclerosis (FSGC) share immunologic and pathogenetic features. We studied 93 Brazilian patients (46 with SSNS and 47 with FSGC) and 104 control subjects with the objective of characterizing the immunogenetic profile of these varieties of idiopathic nephrotic syndrome. HLA-A, -B, and -DR antigens were typed using a complement-dependent microlymphocytotoxicity assay. No significantly association was observed with HLA-A or -B antigens in either group; however, HLA-B7 and -B12 antigens were increased in SSNS patients. HLA-DR7, -DR1 and the combination of HLA-DR1/DR7 antigens were significantly increased in the total group of patients with SSNS compared to controls or to FGSC patients. The study of only Caucasoid individuals revealed the HLA-DR7 antigen remained significantly increased in SSNS patients. The HLA-B7/DR7 haplotype was significantly increased in both SSNS and FSGC patients. Although the Brazilian population is highly miscigenated, the same antigen (HLA-DR7) which confers susceptibility to SSNS in other Caucasian population is still prevalent in this series

Rheumatic fever and HLA antigens

In this study we typed HLA class I and II antigens in a series of patients presenting with the distinct major clinical manifestations of rheumatic fever (RF), i.e, chorea, carditis or arthritis. Ninety-one patients with RF were evaluated for HLA-A, -B and -DR antigens. Thirty-three had pure chorea, 26 pure carditis, 16 pure arthritis, and 16 carditis plus arthritis. HLA-DR1 antigens were overrepresented in the total group of patients with RF and in all the subgroups studied, excluding the chorea subgroup in which the frequency of HLA-DR1 antigen was not increased. The results reported here indicate that immunogenetic susceptibility to RF may vary according to the major clinical manifestations presented by the patient